Figure 1. Effect of poly(I:C) on the expression of MMP-1and MMP-3 in human corneal fibroblasts. A, C: Cells were incubated for 24 h in the presence of the indicated concentrations of poly(I:C), after which the culture supernatants were collected and subjected to immunoblot analysis with antibodies to MMP-1 (A) and to MMP-3 (C). The positions of the bands corresponding to proMMP-1 and proMMP-3 are indicated. Data are representative of three independent experiments. B, D: Cells were incubated for 24 h in the absence or presence of poly(I:C) at 1 µg/ml, after which total RNA was isolated and subjected to RT and real-time PCR analysis of MMP-1 (B) and MMP-3 (D) mRNAs. Data were normalized by the abundance of GAPDH mRNA and are presented in arbitrary units; they are means±SEM from three separate experiments. *p<0.05 (Dunnett’s test) versus the corresponding value for cells cultured without poly(I:C).