Figure 5 of Zhong, Mol Vis 2011; 17:492-507.

Figure 5. Representative images show transgene expression after intravitreous delivery of recombinant adeno-associated virus-green fluorescent protein (AAV-GFP) in the oxygen induced ischemic retinopathy (OIR) model. At postnatal day 7, pups received an intravitreal injection of AAV-GFP vector and were then placed in a 75% oxygen box for 5 days. Thereafter, pups were returned to room air for another 5 days. At day 10 after injection (postnatal day 17), pups were killed. Choroidal flatmounts (A, B) and cryosections (C, D) were prepared. Green fluorescent protein expression was directly observed using fluorescence microscopy. Original magnifications were as follows: A: ×5, B: ×40 C, D: ×20 . The figure shows increased expression of the reporter gene, GFP. The following layers are illustrated: RPE: retinal pigmental epithelium, OS: outer segment, OPL: outer plexiform layer, INL: inner nuclear layer, IPL: inner plexiform layer, GCL: ganglion cell layer.