Figure 3. Increased vasopermeability of retinas from B186 but not from B167 vascular endothelial growth factor. Quantitative blood brain barrier (BRB) assays were performed as described in Methods following subretinal injection of AAV-VEGF-B167, AAV-VEGF-B186, or AAV-green fluorescent protein (GFP) control vectors in C57BL/6J mice. The retina to lung leakage ratio is shown in A; the retina to renal leakage ratio is shown in B. Each bar represents the mean (±SEM) ratio of CPM per milligram of retina to CPM per milligram of lung or kidney. Sample sizes for A: GFP is n=5; VEGF-B167 is n=8; VEGF-B186 is n=7. B: GFP is n=7; VEGF-B167 is n=8; VEGF-B186 is n=9. P-values for the retina to lung leakage ratio relative to GFP were 0.10 for VEGF-B167 and 0.04 for VEGF-B186. P-values for the retina to renal leakage ratio relative to GFP were 0.05 for VEGF-B167 and 0.03 for VEGF-B186, determined by unpaired t test, from the GFP control retinas.