Figure 2. No obvious induction by vascular endothelial growth factor (VEGF)-B167 or 186 of inflammatory response 4 weeks after subretinal injection of adeno-associated virus (AAV)-VEGF-B167 and 186 in C57BL/6J mice. A–C: Hematoxylin and eosin staining; D–G: Leukostasis assay indicated that neither AAV-VEGF-B167 (E, G) nor AAV-VEGF-B186 (F, G) caused significant retinal leukostasis compared to the AAV-GFP control (D). Data are presented as mean±SEM. Sample sizes for GFP and VEGF-B167 are 10 and for VEGF-B186 is 8. For GFP versus VEGF-B167, p=0.13; for GFP versus VEGF-B186, p=0.3. H: An image for comparison showing vascular endothelial growth factor (VEGF)-A-induced leukostasis 6 h after intravitreal administration of 1×10⁻⁶ M VEGF-A. Arrows are pointing to leukocytes adhering to the vessel wall. Original magnification was ×20.