Table 1 of Paliwal, Mol Vis 2011; 17:481-485. Table 1 of Paliwal, Mol Vis 2011; 17:481-485.
Table 1. The VSX1 primer sequences.
Exon |
Forward Primer |
Reverse Primer |
Amplicon size (bp) |
Melting temperature |
|---|---|---|---|---|
| 1 | 5’-CAGCTGATTGGAGCCCTTC-3’ | 5’-CTCAGAGCCTAGGGGACAGG-3’ | 599 | 58 °C |
| 2 | 5’-GCCCACTAAAAATGCAGAA-3’ | 5’-GCCTCCTAGGAACTGCAGAA-3’ | 393 | 59 °C |
| 3 | 5’-CATTCAGAGGTGGGGTGTT-3’ | 5’-TCTTGTGGTGCCTTCAGCTA-3’ | 419 | 62 °C |
| 4 | 5’-GATCATGATCGGGAGAGAAG-3’ | 5’-CGTTGCTTTGCTTTGGAAAT-3’ | 394 | 59 °C |
| 5 | 5’-CCCCAGAGATAGGCACTGAC-3’ | 5’-TGGACAATTTTTGTCTTTTGG-3’ | 495 | 59 °C |