Figure 8. Evaluation of biocompatibility of passage 101 HCE cells on dAMs. A: The confluent cell sheet formed by HCE cells on dAM 116 h after inoculation. The cell density and elongated polygonal morphology were shown. Scale bar: 25 μm. B: alizarin red staining pattern of the HCE cell sheet. The cell density, elongated polygonal morphology and established intercellular junctions were shown. Scale bar: 25 μm. C: SEM image of the HCE cell sheet. The confluent cell sheet and elongated polygonal morphology of HCE cells were shown. Scale bar: 25 μm. D: hematoxylin-eosin (HE) staining pattern of the frozen section of the HCE cell sheet. The confluent monolayer cell sheet formed by HCE cells on dAM was shown. Scale bar: 100 μm. E: TEM image of the HCE cell sheet. The tightly attaching status of the cell sheet and dAM was shown. Scale bar: 4 μm. F: TEM image of the HCE cell sheet. The ultrastructure of HCE cells, the established cell-cell junctions (arrow heads) and cell-dAM junctions (asterisk) were shown. Scale bar: 500 nm.