Figure 1. In vitro culture of HCE cells. A: HCE cells 36 h after plating, showing the non-spread polygonal cell morphology, i.e., corneal endothelial-like morphology. B: The monolayer formed by HCE cells 6 weeks after primary culture initiation, showing the plump polygonal cell morphology. C: Passage 101 HCE cells, showing the co-existence of endothelioid and fibroblast-like morphology. D: Passage 224 HCE cells, showing the co-existence of elongated polygonal and fibroblast-like morphology. Scale bar: 50 μm.