Table 1 of Prommajan, Mol Vis 2011; 17:456-460. Table 1 of Prommajan, Mol Vis 2011; 17:456-460.
Table 1. Primers and PCR conditions for IDUA mutation analysis.
Exon |
Primer name |
Primer sequences for PCR (5′ to 3′) |
Product size (bp) |
Melting temperature (°C) |
|---|---|---|---|---|
| 1 | IDUA-Ex1F | F-ACCCAACCCCTCCCAC | 398 | 58 |
| IDUA-Ex1R | R-AGCTTCAGAGACCGGAG | |||
| 2 | IDUA-Ex2F | F-GAACGTGTGTGTCAGCCG | 304 | 62 |
| IDUA-Ex2R | R-GCTCGGAAGACCCCTTGT | |||
| 3–4 | IDUA-Ex3/4F | F-TTCCAGCCTGGAGCATGGAG | 516 | 62 |
| IDUA-Ex3/4R | R-GTTGCACCCCTATGACGCAG | |||
| 5–6 | IDUA-Ex5/6F | F-TCACCTTGCACCCTCCCTCC | 576 | 62 |
| IDUA-Ex5/6R | R-GCTGACCCTGGTGGTGCTGA | |||
| 7 | IDUA-Ex7F | F-TGCGGCTGGACTACATCTC | 448 | 62 |
| IDUA-Ex7R | R-GCAGCATCAGAACCTGCTACT | |||
| 8 | IDUA-Ex8F | F-CCACCTTCCTCCCGAGAC | 386 | 62 |
| IDUA-Ex8R | R-GGAGCGCACTTCCTCCAG | |||
| 9–10 | IDUA-Ex9F | F-TCCTTCACCAAGGGGAGG | 701 | 58 |
| IDUA-Ex10R | R-TCCTCAGGGTTCTCCAGG | |||
| 11–12 | IDUA-Ex11/12F | F-GTGTGGGTGGGAGGTGGA | 466 | 62 |
| IDUA-Ex11/12R | R-CTTCACCCATGCGGTCAC | |||
| 13–14 | IDUA-Ex13/14F | F-CTGCCTGCTCCCACCTTTGHA | 530 | 62 |
| IDUA-Ex13/14R | R-CCCATGCTGCCCTCCCATCA |