Table 1 of Hu, Mol Vis 2011; 17:436-442. Table 1 of Hu, Mol Vis 2011; 17:436-442.
Table 1. List of the primers and annealing temperatures used for the amplification of the whole exon of the FOXL2 gene containing coding and flanking regions with BPES.
Primer |
Sequence (5′-3′) |
Product length (bp) |
Annealing temperature (°C) |
|---|---|---|---|
| AF | GTGGAGCCCATACGAATCAG | 610 | 62 |
| AR | GTACGAGTACGGGGGCTTCT | ||
| BF | CAGCGCCTGGAGCGGAGAG | 545 | 64 |
| BR | CTTGCCGGGCTGGAAGTGC | ||
| CF | GACCCGGCCTGCGAAGACA | 517 | 66 |
| CR | GGCCGCGTGCAGATGGTGT | ||
| DF | CGCGGCCGCTGTGGTCAAG | 500 | 68 |
| DR | GCTGGCGGCGGCGTCGTC | ||
| EF | CCTCTTTGTCCCCTCAGTTTA | 467 | 51 |
| ER | CGGTGTAAACCGAGTACAGG | ||
| FF | AGAAAGGGACGGACCAATAC | 518 | 55 |
| FR | CAGATAGGGAGAGGGTGAAAC | ||
| GF | GAAGTATTGTGGCCTTGGAGT | 542 | 55 |
| GR | ATTTATTCGGGAATCGACAAG |