Figure 3. Flow cytometric analysis of helper T-cell activation markers in draining lymph nodes. A: Representative FACS plot of activation marker expression on CD4+ single positive T-helper cell in draining LN. Events acquired: 2×10⁵. B: Example of CD25 and CD134 expression pattern on CD4+CD8+ double positive T-cells. Bar diagrams: Cumulative results for the quantification of T-cell activation status of Helper T-lymphocytes. An asterisk (*) indicates statistical significance at p≤0.05 determined by Mann–Whitney U-Test.