Figure 2. Vesicular glutamate transporter 3 amacrine cell synapses that are undetectable in DBA/2J retinas. Representative globe cross-sections immunostained with vesicular glutamate transporter 3 (VGLUT3) antibody show VGLUT3 expression in the retinal inner plexiform layer. Immunostaining shows VGLUT3 (red) expression in the control C57BL/6J (C), and BALB/c (D) retinas, while young nonglaucomatous DBA/2J (A) or old glaucomatous DBA/2J (B) VGLUT3 expression levels are undetectable. Nuclear DAPI staining (blue) is also shown. Merge represents the combination of Blue and Red channels. Expression was assessed in animals ranging from postnatal day 8 to 21 months of age. Immunostaining of DBA/2J (E) and C57BL/6J (F) tissue shows VGLUT3 (red) expression in the brain of both animal strains.