Figure 4. Immunohistochemical staining 48
h after alkali burn at 100× magnification. A, B: The
control group; C, D: the amniotic membrane (AM)
suspension group; E, F: the serum eyedrop group; and G,
H: the comparison group without any manipulation. The figure is
representative of the experiments. Cell nuclei were stained with
4',6-diamidino-2-phenylindole (DAPI; blue; A, C, E,
F), and the F4/80 expressions (red) on the section of cornea 48
h after alkali burn were noted (B, D, F, H)
amount of F4/80 expression was largest in the control group.
However, F4/80 expression is hardly seen in the AM suspension and serum
eyedrop groups. Note that there is no expression of F4/80 in the
comparison group without any manipulation.