Figure 4. Immunohistochemical staining 48 h after alkali burn at 100× magnification. A, B: The control group; C, D: the amniotic membrane (AM) suspension group; E, F: the serum eyedrop group; and G, H: the comparison group without any manipulation. The figure is representative of the experiments. Cell nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI; blue; A, C, E, F), and the F4/80 expressions (red) on the section of cornea 48 h after alkali burn were noted (B, D, F, H) The amount of F4/80 expression was largest in the control group. However, F4/80 expression is hardly seen in the AM suspension and serum eyedrop groups. Note that there is no expression of F4/80 in the comparison group without any manipulation.