Figure 3. Hyperacetylation in retinal ganglion cells after histone deacetylase inhibitor treatment. Representative photographs of anti-acetylated histone proteins (AcH3; green)- and anti-Thy-1.1 (red)- labeled retinal ganglion cells (RGCs) of controls (A-C) and after sodium butyrate (SB) treatment (D-F). Thy-1.1 immunolabeling was used to specifically identify RGCs (white arrows) among other retinal cells (black arrowheads). In comparison to the control, the AcH3 immunoreactivity seems upregulated in RGCs after SB treatment. The scale bar in F is for all pictures and represents 100 µm. The insets show a single RGC at a higher magnification.