Table 1 of Zhang, Mol Vis 2011; 17:380-387. Table 1 of Zhang, Mol Vis 2011; 17:380-387.
Table 1. Primers used in Polymerase Chain Reaction for amplification of TGFBI.
Exon |
Primer direction |
Sequence (5′→3′) |
Annealing temperature (°C) |
Product length (bp) |
|---|---|---|---|---|
| 1 | Forward | GCTTGCCCGTCGGTCGCTA | 64.3 | 234 |
| Reverse | TCCGAGCCCCGACTACCTGA | |||
| 2 | Forward | AGGCAAACACGATGGGAGTCA | 60.1 | 204 |
| Reverse | TAGCACGCAGGTCCCAGACA | |||
| 3 | Forward | CCAGATGACCTGTGAGGAACAGTGA | 60.1 | 232 |
| Reverse | CCTTTTATGTGGGTACTCCTCTCT | |||
| 4 | Forward | TCCTCGTCCTCTCCACCTGT | 58.0 | 353 |
| Reverse | CTCCCATTCATCATGCCCAC | |||
| 5 | Forward | CCTGGGCTCACGAGGGCTGAGAACAT | 60.1 | 387 |
| Reverse | GCCCCTCTTGGGAGGCAATGTGTCCC | |||
| 6 | Forward | CCTGGGCTCACGAGGGCTGAGAACAT | 59.5 | 403 |
| Reverse | GCCCCTCTTGGGAGGCAATGTGTCCC | |||
| 7 | Forward | GTGAGCTTGGGTTTGGCTTC | 63.5 | 387 |
| Reverse | ACCTCATGGCAGGTGGTATG | |||
| 8 | Forward | TGAGGTTATCGTGGAGTG | 52.6 | 435 |
| Reverse | CACATCAGTCTGGTCACA | |||
| 9 | Forward | ACTCACGAGATGACATTCCT | 60.5 | 284 |
| Reverse | TCCAGGGACAATCTAACAGG | |||
| 10 | Forward | TAGAAGATACCAGATGTTAAGG | 56.1 | 426 |
| Reverse | TGTCAGCAACCAGTTCTCAT | |||
| 11 | Forward | CCTGCTACATGCTCTGAACAA | 58.0 | 321 |
| Reverse | GAATCCCCAAGGTAGAAGAAAG | |||
| 12 | Forward | GACTCTACTATCCTCAGTGGTG | 58.0 | 324 |
| Reverse | ATGTGCCAACTGTTTGCTGCT | |||
| 13 | Forward | CATTAGACAGATTGTGGGTCA | 59.9 | 419 |
| Reverse | GGGCTGCAACTTGAAGGTT | |||
| 14 | Forward | GCGACAAGATTGAAACTCCAT | 58.0 | 315 |
| Reverse | CTCTCCACCAACTGCCACAT | |||
| 15 | Forward | CCCTCAGTCACGGTTGTT | 58.0 | 348 |
| Reverse | GGAGTTGCCTTGGTTCTT | |||
| 16 | Forward | CTTGCACAACTTATGTCTGC | 58.0 | 319 |
| Reverse | TGCACCATGATGTTCTTATC | |||
| 17 | Forward | AGTGAAGTTTCACAAACCAC | 58.0 | 475 |
| Reverse | CCACATTTGGGATAGGTC |