Figure 7. Results of statistical analysis of occludin gene expression relative to 18S rRNA in bovine retinal capillary endothelial cells (BRCECs) for 24 h. ADMA downregulated occludin mRNA expression in BRCECs for 24 h. Benazepril, telmisartan, or diphenyliodonium (DPI) upregulated occludin mRNA level. However, N-acetyl-l-cysteine (NAC) had no effect on occludin mRNA level. In group C, BRCECs were cultured in endothelial cell medium (ECM) with 0.4% fetal bovine serum (FBS). In group A, BRCECs were cultured in ECM with 0.4% FBS plus ADMA (100 μM). In group “A+B,” BRCECs were cultured in the same media as group A and 10 μM benazepril. In “A+T,” BRCECs were cultured in the same media as group A and 10 μM telmisartan. In group “A+D,” BRCECs were cultured in the same media as group A and 10 μM DPI. In group “A+N,” BRCECs were cultured in the same media as group A and 10 mM NAC (mean±SD, n=3). *p<0.05 versus group C, #p<0.05 versus group A.