Figure 5. Occludin protein expression in
bovine retinal capillary endothelial cells. A: western blotting
analysis showing the presence of occludin protein in bovine retinal
capillary endothelial cells (BRCECs). B: The results of
statistical analysis of protein level relative to β-actin. Asymmetric
dimethylarginine (ADMA) significantly downregulated occludin protein
expression in BRCECs. Benazepril and telmisartan upregulated protein
expression of occludin in BRCECs exposed to ADMA. However,
diphenyliodonium (DPI) and N-acetyl-l-cysteine (NAC) had no effect on
occludin expression. In group C, BRCECs were cultured in ECM with 0.4%
fetal bovine serum (FBS). In group A, BRCECs were cultured in ECM with
0.4% FBS plus ADMA (100 μM). Group “A+B” represents same as for group A
and 10 μM benazepril. Group “A+T” represents same as for group A and 10
μM telmisartan. Group “A+D” represents same as for group A and 10 μM
DPI. Group “A+N” represents same as for group A and 10 mM NAC (mean±SD,
n=3). **p<0.01 versus group C, ##p<0.01 versus group A.