Figure 5 of Chen, Mol Vis 2011; 17:332-340.


Figure 5. Occludin protein expression in bovine retinal capillary endothelial cells. A: western blotting analysis showing the presence of occludin protein in bovine retinal capillary endothelial cells (BRCECs). B: The results of statistical analysis of protein level relative to β-actin. Asymmetric dimethylarginine (ADMA) significantly downregulated occludin protein expression in BRCECs. Benazepril and telmisartan upregulated protein expression of occludin in BRCECs exposed to ADMA. However, diphenyliodonium (DPI) and N-acetyl-l-cysteine (NAC) had no effect on occludin expression. In group C, BRCECs were cultured in ECM with 0.4% fetal bovine serum (FBS). In group A, BRCECs were cultured in ECM with 0.4% FBS plus ADMA (100 μM). Group “A+B” represents same as for group A and 10 μM benazepril. Group “A+T” represents same as for group A and 10 μM telmisartan. Group “A+D” represents same as for group A and 10 μM DPI. Group “A+N” represents same as for group A and 10 mM NAC (mean±SD, n=3). **p<0.01 versus group C, ##p<0.01 versus group A.