Figure 4. Intercellular adhesion molecule-1 (ICAM-1) protein expression in bovine retinal capillary endothelial cells. A: western blotting analysis showing the presence of ICAM-1 protein in bovine retinal capillary endothelial cells (BRCECs). B: Results of statistical analysis of protein levels relative to β-actin. Asymmetric dimethylarginine (ADMA) markedly upregulated ICAM-1 protein expression in BRCECs. Telmisartan, diphenyliodonium (DPI), or N-acetyl-l-cysteine (NAC) downregulated ICAM-1 protein expression in BRCECs exposed to ADMA. Benazepril had no effect on ICAM-1 expression. In group C, BRCECs wrre cultured in endothelial cell medium (ECM) with 0.4% fetal bovine serum (FBS). In group A, BRCECs were cultured in ECM with 0.4% FBS plus ADMA (100 μM). In group “A+B,” BRCECs were cultured in the same media as A and 10 μM benazepril. In group “A+T,” BRCECs were cultured in the same media as group A and 10 μM telmisartan. In group “A+D,” BRCECs were cultured in the same media as group A and 10 μM DPI. In group “A+N,” BRCECs were cultured in the same media as A and 10 mM NAC (mean±SD, n=3). **p<0.01 versus C, ##p<0.01 versus group A.