Figure 2. Intracellular reactive oxygen species generation in bovine retinal capillary endothelial cells for 24 h, as determined using the fluorescent probe CM-H₂DCFDA. symmetric dimethylarginine (ADMA) increased intracellular reactive oxygen species (ROS) generation in bovine retinal capillary endothelial cells (BRCECs). The increased reactive oxygen species (ROS) production induced by asymmetric dimethylarginine (ADMA) was markedly inhibited by benazepril, telmisartan, diphenyliodonium, or N-acetyl-l-cysteine. In group C, BRCECs were cultured in endothelial cell medium with 0.4% fetal bovine serum. In group A, BRCECs were cultured in endothelial cell medium with 0.4% fetal bovine serum plus ADMA (100 μM). In group “A+B,” BRCECs were cultured in the same media as A and 10 μM benazepril. In group “A+T,” BRCECs were cultured in the same media as A and 10 μM telmisartan. In group “A+D,” BRCECs were cultured in the same media as A and 10 μM diphenyliodonium. In group “A+N,” BRCECs were cultured in the same media as group A and 10 mM N-acetyl-l-cysteine (mean±SD, n=3). **p<0.01 versus group C, ##p<0.01 versus group A.