Figure 5. Quantification of the retinal area with neovascularization in P17 normal air mice and P17 oxygen-induced retinopathy (OIR) mice. The P17 normal air mice (control; A) and P17 OIR mice were given a retro-orbital injection (B) or left-ventricular perfusion (C) of fluorescein isothiocyanate dextran (FITC)-dextran. The neovascularization areas were outlined with Image Pro-Plus 5.1 software (Media Cybernetics Company, Silver Spring, MD). The percentage of the retinal neovascularization in the OIR group is a significant increase when compared to the control group (n=6), and no statistical difference was found between the retro-orbital injection (RO) group (n=6) and the LV group (n=6) D: Data shown are mean±standard deviation, *p<0.05. NV: neovascularization, LV, left-ventricular perfusion. Scale bar: 100 μm.