Figure 5. Iberiotoxin attenuated protective effects of unoprostone and M1 on mouse retinal cone-cell line 661W cell death. A: Unoprostone (1 µM), and M1 (0.1 µM) or iberiotoxin (1 µM) were added 1 h before H₂O₂ treatment. B: Unoprostone (3 µM), M1 (3 µM) or iberiotoxin (1 µM) were added just before light irradiation. The number of cells exhibiting PI fluorescence was counted, and positive cells were expressed as the percentage of PI to Hoechst 33342. Data are expressed as mean±SEM (n=6). ** p<0.01 versus Unoprostone or M1 treatment; ^## p<0.01 versus vehicle (Student’s t-test). Con: control; Veh: vehicle; IbTX: iberiotoxin.