Figure 4. Unoprostone and M1 suppressed phagocytotic dysfunction induced by white light in ARPE-19. A: Representative fluorescence microscopy shows latex beads and morphology, or latex beads and nuclear staining for Hoechst 33342 after 48 h light irradiation. Arrowheads indicate the fluorescent beads. B, C: Unoprostone (0.001–1 µM), M1 (0.001–1 µM), and PGF_2α (1 µM) or latanoprost (1 µM) were added before light irradiation. Latex beads were added after light irradiation, and 4 h later, they were removed by washing. The number of intracellular latex beads was counted, and phagocytotic activity was expressed as the percentage of latex beads to total cell numbers. Data are expressed as mean±SEM (PGF_2α and Lat; n=4, unoprostone and M1; n=8). ** p<0.01 versus vehicle; ^## p<0.01 versus control (Dunnett’s test). Con: control; Veh: vehicle; Lat: latanoprost. Scale bar represents 50 µm.