Figure 3 of Tsuruma, Mol Vis 2011; 17:3556-3565.


Figure 3. Unoprostone reduced morphological change induced by light irradiation in mouse retinal cone-cell line 661W cells. A: Representative images show cell morphologies. Control cells had an elongated appearance with extended processes, whereas cell morphology was altered to a round shape after 24 h light irradiation. Cells were treated with 10 µM of unoprostone and 100 µM of trolox before light irradiation. B: Unoprostone (1–10 µM) was added before light irradiation. The number of nonround-shaped cells was counted, and expressed as the percentage of total cell numbers. C: Representative images show JC-1 stained cells. Healthy cells with mainly JC-1 J-aggregates can be detected as red cells, and unhealthy or apoptotic cells with mainly JC-1 monomers can be detected as green cells. Merged (orange) cells were determined to be pre-apoptotic (early or middle state of transition to cell death) cells. D: The number of cells with each color were counted and expressed as the percentage of total cell numbers (red bar=red fluorescence cells, orange bar=merged cells, and green bar=green fluorescence cells, respectively). Data are expressed as mean±SEM (B; n=6, D; n=3). * p<0.05, ** p<0.01 versus vehicle; ## p<0.01 versus control (Dunnett’s test). Con: control; Veh: vehicle; Tro: trolox. Scale bar represents 100 µm.