Figure 2. Unoprostone and M1 reduced cell death induced by white light in mouse retinal cone-cell line 661W cells. A: Representative fluorescence microscopic images show nuclear staining for Hoechst 33342 and PI after 24 h light irradiation. Unoprostone (0.1–3 µM), M1 (0.1–3 µM), PGF_2α (3 µM), latanoprost (3 µM) or trolox (100 µM) were added before light irradiation. B: The number of cells exhibiting PI fluorescence was counted, and positive cells were expressed as the percentage of PI to Hoechst 33342. Data are expressed as mean±SEM (n=6). ** p<0.01 versus vehicle; ^## p<0.01 versus control (Dunnett’s test). Con: control; Veh: vehicle; Lat: latanoprost; Tro: trolox. Scale bar represents 100 µm.