Figure 1. Unoprostone and M1 suppressed H₂O₂-induced photoreceptor cell death. A: Representative fluorescence microscopic images showing nuclear staining for Hoechst 33342 and PI after 24 h H₂O₂ treatment. Unoprostone (0.01–1 µM), M1 (0.01–1 µM), PGF_2α (1 µM), and latanoprost (1 µM) or trolox (100 µM) were added 1 h before H₂O₂ treatment. B: The number of cells exhibiting PI fluorescence was counted, and expressed as the percentage of PI positive cells to Hoechst 33342 positive cells. Data are expressed as mean±SEM (n=6). * p<0.05, ** p<0.01 versus vehicle; ^## p<0.01 versus control (Dunnett’s test). Con: control; Veh: vehicle; Lat: latanoprost; Tro: trolox. Scale bar represents 100 µm.