Figure 5. E235Q mutant protein is produced and correctly localizes to the pericentriolar region in HeLa cell culture. Immunofluorescence of FLAG-tagged wild-type and mutant BBS4 protein in HeLa cells. Cells were stained using an anti-FLAG antibody (green) for BBS4 expression and anti-γ tubulin antibody (red) for centrosomal localization. Nuclei were stained with DAPI.