Figure 2 of Wankun, Mol Vis 2011; 17:3512-3522.


Figure 2. Paeoniflorin (PF) protects human retinal pigment epithelium cells from H2O2-induced cell death. A: Cytotoxicity of paeoniflorin to ARPE-19 cells were measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide (MTT) assay. ARPE-19 cells were treated with or without different concentrations of PF (5–200 μg/ml) for 24 h, and cell viability was assessed by an MTT assay. The results are expressed as percentage of control, and each value represents the mean±SEM of three independent experiments (n=3 experiments). B: Cell viability of ARPE-19 cells following H2O2 exposure were measured by MTT assay. The cells were treated with or without different concentrations of H2O2 (50–800 μM) for 24 h. Cell viability was measured by an MTT assay. The results are expressed as percentage of control, and each value represents the mean±SEM of three independent experiments (n=3 experiments, *p<0.05). C: Cell viability was measured in ARPE-19 cells treated with 200 μM H2O2 or different concentrations of PF (50–200 μg/ml) by an MTT assay. The results are expressed as percentage of control, and each value represents the mean±SEM of three independent experiments (n=3 experiments, *p<0.05). D: Cell viability was measured using 4', 6-diamidino-2-phenylindole (DAPI) staining. Strong fluorescent spots show apoptotic nuclei. Figures were selected as representative data from three independent experiments. Scale bar, 10 μm.