Table 4 of
He, Mol Vis 2011; 17:3494-3511. Table 4 of
He, Mol Vis 2011; 17:3494-3511.
Table 4. Optimization of immunolocalization for cell cycle-related proteins in endothelial cells on flat- mounted whole human corneas.
| Target protein | Classification | Selected protocols |
|---|---|---|
| Ki67 | best | 0.5% formaldehyde |
| alternatives | 4% formaldehyde; methanol | |
| PCNA | best | 0.5% formaldehyde 60 min at RT + AR with 0.5% SDS 5 min at RT |
| alternatives | 0.5% formaldehyde; methanol | |
| MCM-2 | best | 0.5% formaldehyde |
| alternatives | / | |
| cyclin D1 (abcam 31450) | best | Carnoy I at −20 °C |
| alternatives | 0.5% formaldehyde | |
| cyclin E | best | 0.5% formaldehyde 60 min at RT + AR with 0.1% trypsin 5 min at 37 °C |
| alternatives | 0.5% formaldehyde | |
| cyclin A | best | 0.5% formaldehyde 60 min at RT + AR with 0.5% SDS 5 min at RT |
| alternatives | / | |
| P16 | best | 0.5% formaldehyde for 60 min at RT + AR with 0.5% SDS 5 min at RT |
| alternatives | 0.5% formaldehyde | |
| P21 (cell signaling #2946) | best | 0.5% formaldehyde |
| alternatives | / | |
| P27 | best | 0.5% formaldehyde |
| alternatives | / |