Table 3 of
He, Mol Vis 2011; 17:3494-3511. Table 3 of
He, Mol Vis 2011; 17:3494-3511.
Table 3. Optimization of immunolocalization of ZO-1, actin, hnRNP L, and histone H3 in endothelial cells on flat-mounted whole human corneas.
| Target protein | Classification | Selected protocols |
|---|---|---|
| ZO-1 | best | methanol at −20 °C |
| second choice | methanol at RT; 0.5% formaldehyde at RT; Carnoy I at −20 °C | |
| Actin | best | methanol at RT |
| second choice | Carnoy I at −20 °C | |
| hnRNP L | best | 0.5% formaldehyde at RT |
| second choice | methanol at RT | |
| histone H3 | best | 0.5% formaldehyde + AR with 0.5% SDS in water 5 min at RT * |
| second choice | methanol at RT |