Table 1 of He, Mol Vis 2011; 17:3494-3511.


Table 1. Fixation protocols and antigen retrieval methods assessed on whole corneas.

Number Fixation protocols
1 4% formaldehyde*, in PBS, pH 7.45 30 min at 4 °C
2 0.5% formaldehyde*, in PBS, pH 7.45 30 min at 4 °C
3 4% formaldehyde*, in PBS, pH 7.45 30 min at RT
4 0.5% formaldehyde*, in PBS, pH 7.45 30 min at RT
5 100% methanol 30 min at −20 °C
6 100% methanol 30 min at RT
7 100% acetone 30 min at −20 °C
8 100% acetone/100% methanol (1:1 v/v) 30 min at −20 °C
9 100% acetone/100% methanol (1:1 v/v) 30 min at RT
10 Carnoy I** 30 min at −20 °C
11 Carnoy I** 30 min at RT
12 Bouin modified*** 45 min at RT
13 0.5% Zn(OAc)2 + 0.5% ZnCl2 + 0.05% Ca(OAc)2 in 0.1M Tris base buffer 45 min at RT (Tris-Zinc)
14 HgCl2 6 g in formalin (37%–40%) 10 ml + distilled water 90 ml 45 min at RT
15 ZnCl2 0.16 g + PFA 4 g + NaCl 5 g in distilled water 100 ml 45 min at RT
Number Antigen retrieval methods
1 0.1 M citrate buffer pH 6, 4 h at 55 °C§
2 0.01 M EDTA buffer pH 10, 4 h at 55 °C§
3 0.1% trypsin in PBS, 5 min at 37 °C
4 4 M urea in water, 4 h at RT
5 0.5% sodium dodecyl sulfate in water, 5 min at RT