Table 1 of
He, Mol Vis 2011; 17:3494-3511. Table 1 of
He, Mol Vis 2011; 17:3494-3511.
Table 1. Fixation protocols and antigen retrieval methods assessed on whole corneas.
| Number | Fixation protocols |
|---|---|
| 1 | 4% formaldehyde*, in PBS, pH 7.45 30 min at 4 °C |
| 2 | 0.5% formaldehyde*, in PBS, pH 7.45 30 min at 4 °C |
| 3 | 4% formaldehyde*, in PBS, pH 7.45 30 min at RT |
| 4 | 0.5% formaldehyde*, in PBS, pH 7.45 30 min at RT |
| 5 | 100% methanol 30 min at −20 °C |
| 6 | 100% methanol 30 min at RT |
| 7 | 100% acetone 30 min at −20 °C |
| 8 | 100% acetone/100% methanol (1:1 v/v) 30 min at −20 °C |
| 9 | 100% acetone/100% methanol (1:1 v/v) 30 min at RT |
| 10 | Carnoy I** 30 min at −20 °C |
| 11 | Carnoy I** 30 min at RT |
| 12 | Bouin modified*** 45 min at RT |
| 13 | 0.5% Zn(OAc)2 + 0.5% ZnCl2 + 0.05% Ca(OAc)2 in 0.1M Tris base buffer 45 min at RT (Tris-Zinc) |
| 14 | HgCl2 6 g in formalin (37%–40%) 10 ml + distilled water 90 ml 45 min at RT |
| 15 | ZnCl2 0.16 g + PFA 4 g + NaCl 5 g in distilled water 100 ml 45 min at RT |
| Number | Antigen retrieval methods |
| 1 | 0.1 M citrate buffer pH 6, 4 h at 55 °C§ |
| 2 | 0.01 M EDTA buffer pH 10, 4 h at 55 °C§ |
| 3 | 0.1% trypsin in PBS, 5 min at 37 °C |
| 4 | 4 M urea in water, 4 h at RT |
| 5 | 0.5% sodium dodecyl sulfate in water, 5 min at RT |