Figure 2. Optimization of immunolocalization of ZO-1, actin, hnRNP L, and histone H3 in endothelial cells on flat-mounted whole human corneas. Only the best combination is shown in the first line, compared with the 4% formaldehyde usually considered as the gold standard in immunohisto- and cytochemistry. Original magnification 40×. Bar 10 μm.