Table 1 of Lacassagne, Mol Vis 2011; 17:309-322.
Exon | Primers | Sequences (5′-3′) | Size | PCR Conditions |
---|---|---|---|---|
1 | 1F | CCGTTGCTTTGAGCAGATT | 265 | MgCl2 2 mM, dNTP 0,125 µM, Primers 0,1 µM, DMSO 7,5%, TouchDown PCR 62–58 °C |
1R | AAGGCCTCAAAGCCCCAG | |||
2 | 2F | CAGGGCCTCTGATCCCTAC | 341 | MgCl2 1 mM, dNTP 0,2 mM, Primers 1 µM, annealing temperature 56 °C |
2R | GTGAACTGGTACACTGGCCC | |||
3 | 3F | GGGACAGTCTCAGCC ATCTC | 238 | MgCl2 1 mM, dNTP 0,2 mM, Primers 1 µM, annealing temperature 59,5 °C |
3R | CAGCTCCTCGTGATCCTCC | |||
4 | 4aF | CGCTCGCAGCAGAAAGCT | 305 | MgCl2 1 mM, dNTP 0,125 mM, Primers 0,25 µM, annealing temperature 57 °C |
4aR | TGTAGACTGCGGTGCTGAG | |||
4bF | GGCTTCTACGTGACGCTGGT | 317 | MgCl2 1 mM, dNTP 0,125 mM, Primers 0,25 µM, annealing temperature 57 °C | |
4bR | TCCACCCATCTTCCATTC | |||
5 | 5F | ATCCCTTCTGCAGGTTCTCC | 274 | MgCl2 1,5 mM, dNTP 0,2 mM, Primers 0,1 µM, DMSO 5%, annealing temperature 56 °C |
5R | AAACCTTGTTTCCTGTGGACC | |||
6 | 6F | GGGCAGGTGGTGTTCAGA | 181 | MgCl2 1 mM, dNTP 0,2 mM, Primers 1 µM, annealing temperature 58 °C |
6R | CCTTGGTCCTTCTAGCCTCAG | |||
7 | 7F | CATCCTGATTTCAGGGTT CC | 266 | MgCl2 2 mM, dNTP 0,125 µM, Primers 0,1 µM, DMSO 7,5%, TouchDown PCR 62–58 °C |
7R | CTCTGGCCATGCCTCCAG | |||
8 | 8F | AGCTGAGGTTTAAAGGGGGA | 215 | MgCl2 1 mM, dNTP 0,125 mM, Primers 0,25 µM, DMSO 5%, annealing temperature 56 °C |
8R | TCTCTTTGGGTCCACTTTGG | |||
9 | 9F | ACATACAAGGTCCTGCCTGG | 298 | MgCl2 2 mM, dNTP 0,125 mM, Primers 0,1 µM TouchDown PCR 62–58 °C |
9R | GCATTAACTAGTGCTATTCTAAGTTCC | |||
10 | 10aF | GGTGTTGGTCCTTTGTCCAC | 591 | MgCl2 1,5 mM, dNTP 0,125 mM, Primers 0,25 µM, DMSO 5%, TouchDown PCR 62–58 °C |
10aR | CTCTGGCATATCCTCAGGT | |||
10bF | CTTCAAGTCTGCCCCACTGT | 457 | MgCl2 1,5 mM, dNTP 0,125 mM, Primers 0,25 µM, DMSO 5%, TouchDown PCR 62–58 °C | |
10bR | TAGGCTCAGAGCAAGGGAAG | |||
11 | 11F | CATTTTGGTATTTGAAATGAAGG | 216 | MgCl2 1,5 mM, dNTP 0,125 mM, Primers 0,25 µM, annealing temperature 54 °C |
11R | CCATTTGATTCAGGCTGTTGG |