Table 1 of Lacassagne, Mol Vis 2011; 17:309-322.


Table 1. Primers and PCR conditions used for amplifying each exon of the BEST1 gene

Exon Primers Sequences (5′-3′) Size PCR Conditions
1 1F CCGTTGCTTTGAGCAGATT 265 MgCl2 2 mM, dNTP 0,125 µM, Primers 0,1 µM, DMSO 7,5%, TouchDown PCR 62–58 °C
1R AAGGCCTCAAAGCCCCAG
2 2F CAGGGCCTCTGATCCCTAC 341 MgCl2 1 mM, dNTP 0,2 mM, Primers 1 µM, annealing temperature 56 °C
2R GTGAACTGGTACACTGGCCC
3 3F GGGACAGTCTCAGCC ATCTC 238 MgCl2 1 mM, dNTP 0,2 mM, Primers 1 µM, annealing temperature 59,5 °C
3R CAGCTCCTCGTGATCCTCC
4 4aF CGCTCGCAGCAGAAAGCT 305 MgCl2 1 mM, dNTP 0,125 mM, Primers 0,25 µM, annealing temperature 57 °C
4aR TGTAGACTGCGGTGCTGAG
4bF GGCTTCTACGTGACGCTGGT 317 MgCl2 1 mM, dNTP 0,125 mM, Primers 0,25 µM, annealing temperature 57 °C
4bR TCCACCCATCTTCCATTC
5 5F ATCCCTTCTGCAGGTTCTCC 274 MgCl2 1,5 mM, dNTP 0,2 mM, Primers 0,1 µM, DMSO 5%, annealing temperature 56 °C
5R AAACCTTGTTTCCTGTGGACC
6 6F GGGCAGGTGGTGTTCAGA 181 MgCl2 1 mM, dNTP 0,2 mM, Primers 1 µM, annealing temperature 58 °C
6R CCTTGGTCCTTCTAGCCTCAG
7 7F CATCCTGATTTCAGGGTT CC 266 MgCl2 2 mM, dNTP 0,125 µM, Primers 0,1 µM, DMSO 7,5%, TouchDown PCR 62–58 °C
7R CTCTGGCCATGCCTCCAG
8 8F AGCTGAGGTTTAAAGGGGGA 215 MgCl2 1 mM, dNTP 0,125 mM, Primers 0,25 µM, DMSO 5%, annealing temperature 56 °C
8R TCTCTTTGGGTCCACTTTGG
9 9F ACATACAAGGTCCTGCCTGG 298 MgCl2 2 mM, dNTP 0,125 mM, Primers 0,1 µM TouchDown PCR 62–58 °C
9R GCATTAACTAGTGCTATTCTAAGTTCC
10 10aF GGTGTTGGTCCTTTGTCCAC 591 MgCl2 1,5 mM, dNTP 0,125 mM, Primers 0,25 µM, DMSO 5%, TouchDown PCR 62–58 °C
10aR CTCTGGCATATCCTCAGGT
10bF CTTCAAGTCTGCCCCACTGT 457 MgCl2 1,5 mM, dNTP 0,125 mM, Primers 0,25 µM, DMSO 5%, TouchDown PCR 62–58 °C
10bR TAGGCTCAGAGCAAGGGAAG
11 11F CATTTTGGTATTTGAAATGAAGG 216 MgCl2 1,5 mM, dNTP 0,125 mM, Primers 0,25 µM, annealing temperature 54 °C
11R CCATTTGATTCAGGCTGTTGG