Molecular Vision: Lacassagne, Mol Vis 2011; 17:309-322. Table 1

Table 1. Primers and PCR conditions used for amplifying each exon of the BEST1 gene

Exon	Primers	Sequences (5′-3′)	Size	PCR Conditions
1	1F	CCGTTGCTTTGAGCAGATT	265	MgCl₂ 2 mM, dNTP 0,125 µM, Primers 0,1 µM, DMSO 7,5%, TouchDown PCR 62–58 °C
1	1R	AAGGCCTCAAAGCCCCAG	265
2	2F	CAGGGCCTCTGATCCCTAC	341	MgCl₂ 1 mM, dNTP 0,2 mM, Primers 1 µM, annealing temperature 56 °C
2	2R	GTGAACTGGTACACTGGCCC	341
3	3F	GGGACAGTCTCAGCC ATCTC	238	MgCl₂ 1 mM, dNTP 0,2 mM, Primers 1 µM, annealing temperature 59,5 °C
3	3R	CAGCTCCTCGTGATCCTCC	238
4	4aF	CGCTCGCAGCAGAAAGCT	305	MgCl₂ 1 mM, dNTP 0,125 mM, Primers 0,25 µM, annealing temperature 57 °C
	4aR	TGTAGACTGCGGTGCTGAG	305
	4bF	GGCTTCTACGTGACGCTGGT	317	MgCl₂ 1 mM, dNTP 0,125 mM, Primers 0,25 µM, annealing temperature 57 °C
	4bR	TCCACCCATCTTCCATTC	317
5	5F	ATCCCTTCTGCAGGTTCTCC	274	MgCl₂ 1,5 mM, dNTP 0,2 mM, Primers 0,1 µM, DMSO 5%, annealing temperature 56 °C
5	5R	AAACCTTGTTTCCTGTGGACC	274
6	6F	GGGCAGGTGGTGTTCAGA	181	MgCl₂ 1 mM, dNTP 0,2 mM, Primers 1 µM, annealing temperature 58 °C
6	6R	CCTTGGTCCTTCTAGCCTCAG	181
7	7F	CATCCTGATTTCAGGGTT CC	266	MgCl₂ 2 mM, dNTP 0,125 µM, Primers 0,1 µM, DMSO 7,5%, TouchDown PCR 62–58 °C
7	7R	CTCTGGCCATGCCTCCAG	266
8	8F	AGCTGAGGTTTAAAGGGGGA	215	MgCl₂ 1 mM, dNTP 0,125 mM, Primers 0,25 µM, DMSO 5%, annealing temperature 56 °C
8	8R	TCTCTTTGGGTCCACTTTGG	215
9	9F	ACATACAAGGTCCTGCCTGG	298	MgCl₂ 2 mM, dNTP 0,125 mM, Primers 0,1 µM TouchDown PCR 62–58 °C
9	9R	GCATTAACTAGTGCTATTCTAAGTTCC	298
10	10aF	GGTGTTGGTCCTTTGTCCAC	591	MgCl₂ 1,5 mM, dNTP 0,125 mM, Primers 0,25 µM, DMSO 5%, TouchDown PCR 62–58 °C
	10aR	CTCTGGCATATCCTCAGGT	591
	10bF	CTTCAAGTCTGCCCCACTGT	457	MgCl₂ 1,5 mM, dNTP 0,125 mM, Primers 0,25 µM, DMSO 5%, TouchDown PCR 62–58 °C
	10bR	TAGGCTCAGAGCAAGGGAAG	457
11	11F	CATTTTGGTATTTGAAATGAAGG	216	MgCl₂ 1,5 mM, dNTP 0,125 mM, Primers 0,25 µM, annealing temperature 54 °C
11	11R	CCATTTGATTCAGGCTGTTGG	216