Figure 8. Effects of 17β-estradiol on
IL-1β–induced MAPK and IκB-α phosphorylation in corneal
fibroblasts. Cells were incubated in the absence or presence of
17β-estradiol (100 μM) for 12 h and then in the additional
absence or presence of IL-1β (0.1 ng/ml) for 30 min. Cell
lysates were then prepared and subjected to immunoblot analysis
with antibodies to total or phosphorylated (P-) forms of ERK,
JNK, p38 MAPK, or IκB-α. Data are representative of three
independent experiments.