Figure 6. Lack of effect of 17β-estradiol or progesterone on the proliferation of corneal fibroblasts. Cells were cultured for 24 h in MEM and in the absence (negative control) or presence of various concentrations of 17β-estradiol or progesterone or of 10% FBS (positive control). Cell proliferation was evaluated by measurement of BrdU incorporation with an enzyme-linked immunosorbent assay. Data are means±SEM of quadruplicates from an experiment that was repeated three times with similar results. *p<0.05 versus negative control (Dunnett’s test).