Figure 6. Relative expression ratio of the target gene versus the housekeeping gene (glyceraldehyde 3-phosphate dehydrogenase). The ratio for the vertical axis is relative expression ratio of the target gene versus the house-keeping gene (glyceraldehyde 3-phosphate dehydrogenase [GAPDH]). Three independent experiments were conducted. Bars represent standard deviation (SD), and the data were shown as mean±SD. The asterisk indicates p<0.05 in comparison between each rapamycin group and the scratch group with independent samples t-test. Pound sign (#) indicates p<0.05 in comparison between the scratch group and the no-scratch group with independent samples t-test. a, scratch group; b₁, 10 ng/ml rapamycin group; b₂, 100 ng/ml rapamycin group; b₃, 1,000 ng/ml rapamycin group; c, no-scratch group.