Figure 2. Immunofluorescence images. A: In the no-scratch group, after cell confluency, vascular endothelial (VE)-cadherin yielded continuous, linear staining around the periphery of EA.hy926 cells (confocal microscopy 200×). B: In the no-scratch group, after cell confluency, there was no expression of vimentin protein (confocal microscopy 100×). C: In the no-scratch group, after cell confluency, there was no expression of the Twist protein (confocal microscopy 100×). D: In the scratch group, the VE-cadherin expression of the endothelial cells that migrated into the scratch wound was decreased at endothelial cell-cell junctions and was mainly localized to the cytoplasm; VE-cadherin yielded continuous, linear staining around the periphery of EA.hy926 cells on the outside of the scratch wound (confocal microscopy 100×). E: In the scratch group, the vimentin expression of endothelial cells that migrated into the scratch wound (confocal microscopy 100×). F: In the scratch group, the Twist expression of endothelial cells that migrated into the scratch wound (confocal microscopy 100×).