Figure 3. Western blot detect the protein expression of TLR2 and TLR4. A: western blot analyses detect the expression of TLR2 and TLR4 at the protein level in HCEC under stimulation of high glucose and high mannitol. Equal amounts of proteins were loaded. B: Column diagrams and Bars represent the ratio of the scanned immunoblots of TLR2 and TLR4 to that of GAPDH. Untreated HCEC was regarded as control. Data are the mean±SEM of triplicates from an experiment that was repeated three times with similar results. *represent a p<0.05 versus control.