Figure 4. HA significantly decreased BAC-induced cell apoptosis. A: No significant differences in apoptosis of HCEs cells were found at concentrations ranging from 0.00005% to 0.0005%. 0.001% BAC showed a significant increase in cell apoptosis, whereas 0.2% HA was significantly decreased 0.001% BAC-induced cell apoptosis. Cell apoptosis in 0.2% HA and 0.001% BAC co-incubated group showed a significantly increase compared to the control cells. B: Representative images of cell apoptosis examined by the flow cytometry. Differences were significant at p<0.001 (three asterisks) compared to control cells, and p<0.01 (two hash marks) compared between cells treated with BAC alone (BAC/HA^-) and cells treated with a combination of 0.2% HA and BAC (BAC/HA⁺).