Molecular Vision: Venters, Mol Vis 2011; 17:3347-3363. Table 1

Table 1. Summary of labeling the optic cup lip (OCL) or optic cup.

Target	Total embryos	Age range (somite number)	Age range (HH stages)	Reincubation range (days)	Label retained in OCL	Radial	Discrete label
OCL	40	18–33	12+ to 18	1–15	37/40 (92.5%)	36/40 (90%)	1/40*
Optic Cup	20	23–35		1.5–4	0/20	0/20	20/20

The majority of OCL embryos (32/40) were labeled at ≥HH14. Those embryos that had not retained label in the OCL at the end of the re-incubation period were HH15–16 at labeling. The summarized data are the result of injecting >500 embryos. All embryos with any GFP expression were scored, in any particular injection run typically 5% of embryos were infected. *1 embryo had GFP expression in a few cells immediately back from the OCL.