Figure 6 of Venters, Mol Vis 2011; 17:3347-3363.


Figure 6. An anterior-posterior maturation gradient is maintained through development of the iris from the OCL. A, C-I: Transverse sections through E14 eyes. A: A section labeled with Pax3 and MF20. MF20 is absent from the mesenchyme closest to the OCL (bracket). Pax3/MF20 are expressed in the same cells at the anterior position in the skeletal muscle (arrows). B: A confocal image of a wholemount E16 eye labeled with Pax6 and MF20. Bracket indicates a Pax only zone in the eye anterior to musculature. C: A section labeled with MF20. The bar indicates the iris sphincter muscle and the anterior most 100 µm. D-I: Sections through the eyes of embryos exposed to BrdU for 4 h (D-F) or 8 h (G-I) before harvesting. Skeletal muscle is labeled with MF20 (D, F, G, I) and arrows indicate myonuclei that have incorporated BrdU (E, F, H, I). J: Graphic summary of BrdU pulse data. There is a significant difference in the percent of nuclei that have incorporated BrdU comparing the anterior and posterior muscle after an 8 h (p<0.01) but not 4 h (p>0.05) pulse. Scale Bars: 100 µm, * indicates anterior of cup/anterior of pupillary margin). L; lens, OCL; Optic Cup Lip.