Figure 4. Effect of different concentrations of Bevacizumab on BCE cell death as quantified by flow cytometry. Flow cytometry analysis of BCE cells treated with Bevacizumab and/or VEGF. Cells were stained with annexin V/propidium iodide (PI) before flow cytometry analysis. Percentages of annexin V/PI negative (viable cells) are displayed in the lower left quadrant; annexin V negative/PI positive cells are displayed in the upper left quadrant. Percentages of annexin V positive/PI positive (necrotic) cells are displayed in the upper right quadrant. Percentages of annexin V positive/ PI negative (early apoptotic) are displayed in the lower right quadrant. Cells under serum-starved conditions were considered as positive controls. BEV (Bevacizumab); V (vascular endothelial growth factor; VEGF).