Figure 6. Western blot analysis
examined the changes in selected protein expression in RGC-5
cells. Protein lysates from RGC-5 cells were probed with
antibodies to Bdnf, Ntf3, Snap25, Nefl, and GAPDH. Optical
densitometric analysis was performed to calculate the relative
protein amounts of siRNA-treated cells compared to Blank cells.
In the sihoptineurin-3 transfected RGC-5 cells, the expression
of Bdnf was 2.06-fold, Ntf3 was 1.28-fold, Snap25 was
3.119-fold, and Nefl was 2.16-fold lower than that of Blank
cells, respectively (p<0.05; *p<0.05, **p<0.01) n=3.