Figure 4. Electron microscopy showed apoptosis in RGC-5 cells after stable transfection of sihoptineurin-3. A: Nuclear heterochromatin margination, partial membrane dissolution, rough endoplasmic reticulum expansion, and mitochondrial reduction were observed in sihoptineurin-3 RGC-5 cells. B: Mitochondrial outer membrane damage, cell membrane partial dissolution, and apoptotic bodies were observed in sihoptineurin-3 RGC-5 cells. C, D: In sihoptineurin-NC RGC-5 and blank cells, we observed nuclear membrane integrity, with organelles enriched in cytoplasm.