Figure 1. Effects of optineurin siRNA on optineurin expression in transfected cells. A: Quantitative real-time PCR was used to measure optineurin mRNA expression. Mock transfected cells were set to 100%. Optineurin siRNA no. 3 was the most effective in inhibiting optineurin mRNA expression and showed 14.34% of expression in Blank cells (p<0.01, n=3). GAPDH served as the endogenous control. B: western blot analysis was used to measure the amount of optineurin protein in sihoptineurin-1(1), sihoptineurin-2(2), sihoptineurin-3(3), sihoptineurin-4(4), sihoptineurin-NC(5) transient transfected and untransfected PC12 cells. The ratio of inhibition for sihoptineurin-3 was 83.1% (p<0.01, n=3). C: western blot analysis showed the knockdown of optineurin in sihoptineurin-3 stable transfected RGC-5 cells and stable transfected PC12 cells. Western blot showed that the inhibiting ratio of optineurin was 84.96±0.88% of Blank cells (p<0.01, n=3). GAPDH served as the loading control. (*p<0.05, **p<0.01, n=3).