Figure 2. The currents evoked by acid in the rat cultured retinal ganglion cells using the patch-clamp technique. A: The retinal ganglion cells (RGCs) were able to trigger action potentials when stimulated. B: The typical current evoked by pH 5.0. This current could be blocked significantly by amiloride (100 µM) while it was not sensitive to 20 nM PcTx1, a specific antagonist of homomeric ASIC1a channels. Furthermore, the current was not evoked by GMQ, an agonist of homomeric ASIC3 channels. C: The amplitude of the current evoked by pH 6.0 changed during ischemia induced by NaCN treatment. The peak amplitude of current induced by pH 6.0 was potentiated after 5 min’ treatment with 100 µM NaCN (25±0.8% increase in the pH-6.0-induced current before NaCN treatment; n=5). D: Quantitative analysis of the peak amplitude of current induced by pH 6.0 before and after NaCN treatment. Data are mean±SEM (n=5 for each group). The asterisk indicates a p<0.05, compared with the ratio of control group.