Figure 1. The presence of acid-sensing ion channel 1a in retinal ganglion cells in rat-cultured retinal ganglion cells and retinal slices using immunohistochemistry. A: Cultured retinal ganglion cells (RGCs) were identified routinely from other cell types on the basis of soma diameter (≥10 µm). Acid-sensing ion channel (ASIC) 1a immunoreactivity was colabeled with anti-DNA-binding protein that identifies most mature neuronal populations (NeuN) fluorescence. B: ASIC1a immunoreactivity was detected in rat retinal slices including the RGC layer, the inner segment of cone photoreceptors, the inner nuclear layer, the inner plexiform layer, and slightly in the outer plexiform layer. C: Positive control reactions of ASIC1a were performed using rat cortex slices. D: A negative control group for immunohistochemistry studies was conducted by removal of primary antibodies.