Figure 1. Endogenous arginine vasopressin–positive cells in the arginine vasopressin–eGFP transgenic rat retina. Arginine vasopressin (AVP)/eGFP-positive cells in (A-C) the ganglion cell layer (GCL) and (D-F) inner nuclear layer (INL) of the AVP-eGFP transgenic rat retina in a whole-mount preparation. A: AVP-eGFP expression in the GCL under a fluorescence microscope. B: A cell with GFP in the GCL detected by a confocal microscope. C: The GFP-positive cell in B was also positive for AVP. D: AVP-eGFP expression in the INL under a fluorescence microscope. E: Cells with GFP in the INL, detected by a confocal microscope. There are two distinct types of GFP-positive cells: brighter cells and faint cells. Asterisks show faint cells. F: The brighter GFP-positive cell in E was also positive for AVP. The scale bars in A and D are 50 μm. The scale bars in B, C, E, and F are 10 μm. G: Density recovery profile (DRP) of GFP-positive cells in the GCL and brighter GFP-positive cells in the INL (n=7 retina pieces). Error bars indicate standard error of the mean (SEM).