Table 1 of
Rose, Mol Vis 2011; 17:3243-3253. Table 1 of
Rose, Mol Vis 2011; 17:3243-3253.
Table 1. Primer sequences used to amplify the MYOC gene in fragments of <300 base pairs along with primer name, annealing temperature (Ta) for each primer pair and the expected product size.
| Primer name |
Sequence |
Ta (degree Celsius) |
Product Size (base pairs) |
|---|---|---|---|
| RR3 | Forward-5’-TGGATTAAGTGGTGCTTC-3' | 58 | 227 |
| RR4 | Reverse-5’-TGGCTGATGAGGTCATAC-3’ | ||
| RR5 | Forward-5’-GGATGTCCGCCAGGTTT-3’ | 60 | 260 |
| RR6 | Reverse-5’-CAATGTCCGTGTAGCCAC-3’ | ||
| RR7 | Forward-5’-TGGCTACCACGGACAGTT-3’ | 62 | 243 |
| RR8 | Reverse-5’-GAGGTGTAGCTGCTGAC-3’ | ||
| RR9 | Forward-5’-CCTTCATCATCTGTGGCA-3’ | 64 | 248 |
| RR10 | Reverse-5’-GTACAGCTTGGAGGCTT-3’ | ||
| RR1 | Forward-5’-AGAGCTTTCCAGAGGAAG-3’ | 64 | 248 |
| RR2 | Reverse-5’-ATGACTGACATGGCCTGG-3’ | ||
| RR11 | Forward-5’-GTCCCAATGAATCCAGCT-3’ | 62 | 268 |
| RR12 | Reverse-5’-TTGCTGTAGGCAGTCTCC-3’ | ||
| RR13 | Forward-5’-GACCAGCTGGAAACCCA-3’ | 60 | 256 |
| RR14 | Reverse-5’-TGCTGAACTCAGAGTCC-3’ | ||
| RR15 | Forward-5’-CATAGTCAATCCTTGGGC-3’ | 64 | 231 |
| RR16 | Reverse-5’-TAAAGACCACGTGGGCAC-3’ |