Figure 4. Rhodopsin and NinaA show improper localization in heterozygous ninaE mutants. Dark-reared fly retinas were isolated and triple stained for F-actin, Rhodopsin (Rh1), and neither inactivation nor afterpotential protein (NinaA). A-D: Wild-type photoreceptors show proper rhodopsin localization to the base of the rhabdomeres and NinaA is localized to the cell body. E-H: ninaE^R11/+ photoreceptors show partial proper rhodopsin localization with multiple rhodopsin- and ninaA-positive puncta present in the cell body. NinaA staining is restricted to the edges of the cell body. I-L: ninaE^R12/+ photoreceptors show rhodopsin localization to the base of the rhabdomeres, with diffuse rhodopsin staining in the cell body having few distinct rhodopsin-positive vesicles. NinaA stains diffusely throughout the cell body similar to the wild type. Scale bar represents 5 μm.