Figure 2. Heterozygous ninaE mutants show loss of deep pseudopupil. At least 15 newly eclosed ninaE^R11/+ and ninaE^R12/+ flies were reared in the dark for 5 days and deep pseudopupil (DPP) was assayed once per day. A graded scoring system was used wherein scores of 5, 3, or 0 were given to flies with intact, diffuse, or absent DPP, respectively. Note the age-dependent gradual loss of DPP in heterozygous mutant flies. The DPP loss in both ninaE^R11/+ and ninaE^R12/+ flies is statistically significant for all time points (p<0.05).