Molecular Vision: Baiula, Mol Vis 2011; 17:3208-3223. Table 1

Table 1. Inhibitory effect of mapracorat and dexamethasone on the release of interleukin-6, interleukin-8, TNF-α, and CCL5/RANTES induced by ionomycin in human HMC-1 cells.

Cytokine or chemokine assayed	Mapracorat IC₅₀ (μM)	Dexamethasone IC₅₀ (μM)
Interleukin-6	0.141^a (0.053–0.320)^b	0.093 (0.045–0.165)
Interleukin-8	0.063 (0.039–0.164)	0.060 (0.025–0.117)
TNF-α	0.167 (0.041–0.182)	0.144 (0.056–0.298)
CCL5/RANTES	0.086 (0.044–0.398)	0.116 (0.061–0.597)

The effect of mapracorat on secretion of the reported cytokines and chemokines, 18 h after ionomycin (2 μM) stimulation of HMC-1 cells, was determined in comparison to dexamenthasone. Compounds (0.001–10 μM) were added 45 min before ionomycin. Values were calculated by analyzing at least three separated experiments performed in duplicate, where concentration-response curves were measured. ^aFor each cytokine or chemokine assayed, mapracorat did not cause any significant change of the concentration-response curve in comparison to dexamethasone (two-way ANOVA with Bonferroni post-test). ^b95% Confidence limits.